Stahl, E. C. & Brown, B. N. Kupffer cell subsets differ between young and aged murine livers. J. Immunol. 196(1 Supplement), 126.131 (2016). Nucleotide release kinetics of the fluorescent nucleotide analogue MABA-ADP (Jena Bioscience, cat. # NU-893-MNT) and nucleotide binding of MABA-ATP (Jena Bioscience, cat. # NU-806-MNT) were performed in a Perkin Elmer LS55 fluorescence spectrometer instrument (excitation 360 nm, emission 420 nm). These nucleotide analogues carry a fluorescent MANT group attached to the ribose, which shows a significant increase of fluorescence upon Hsp70 binding 31. Wisniewska, M. et al. Crystal structures of the ATPase domains of four human Hsp70 isoforms: HSPA1L/Hsp70-hom, HSPA2/Hsp70-2, HSPA6/Hsp70B’, and HSPA5/BiP/GRP78. PLoS. ONE. 5, e8625 (2010). The thing in the parens (which on some systems may be a combination of letters and numbers, but most often is just a single digit) refers to a section in the manual. "The manual" is the combined volume of manuals available.

In the latest episode of BBC Radio Manchester's The Devils' Advocate podcast, co-hosts Gaz Drinkwater and Amber Haque discuss Alejandro Garnacho's unbelievable overhead finish in Manchester United's 3-0 win at Everton.Manchester United are hoping to strengthen in four positions in January, including in defensive midfield, with concerns over 27-year-old Morocco midfielder Sofyan Amrabat's season so far. (Guardian) , external Palgi, M., Greco, D., Lindstrom, R., Auvinen, P. & Heino, T. I. Gene expression analysis of Drosophila Manf mutants reveals perturbations in membrane traffic and major metabolic changes. BMC Genom. 13, 134 (2012).

Villeda, S. A. et al. Young blood reverses age-related impairments in cognitive function and synaptic plasticity in mice. Nat. Med. 20, 659–663 (2014). Fergus: Oh my lord, what a goal from Garnacho. That is the goal of the season, surely. We were the better team and even though Everton needed the win, we took the points very, very nicely. All the goals were outstanding finishes and our season has just begun. White, R. R. et al. Comprehensive transcriptional landscape of aging mouse liver. BMC Genom. 16, 899 (2015).In the latest episode of BBC Radio Manchester's The Devils' Advocate podcast, co-hosts Gaz Drinkwater and Amber Haque discuss Manchester United's upcoming fixtures at Galatasaray in the Champions League and at Newcastle in the Premier League. ER calcium depletion has been associated with enhanced secretion of MANF protein. However, even under such conditions, a substantial pool of MANF is found in cell lysates 10. This feature was conserved in CHO-K1 cells (Fig. 1d and Supplementary Fig. 1c) and suggested that, in addition to whatever role secreted MANF might have as a factor involved in intercellular communication, an intracellular role for MANF should also be considered. Walker, D. G. & Lue, L. F. Immune phenotypes of microglia in human neurodegenerative disease: challenges to detecting microglial polarization in human brains. Alzheimers Res. Ther. 7, 56 (2015). BUGS top https://gitlab.com/man-db/man-db/-/issues https://savannah.nongnu.org/bugs/?group=man-db COLOPHON top

I think, me as a player I love playing [in these] kind of stadiums because it's a big boost for you. MANF is involved in multiple hepatic metabolic diseases as a secreted protein, including virus- or alcohol-induced liver injury and cancer, through interacting with XBP1 [ 65– 69]. In detail, the transcription of MANF is enhanced by XBP1 activation [ 69]. MANF also regulates its expression through binding to XBP1s. Moreover, the role of MANF from different sources in liver disease is different. Immune cell-derived MANF protects against liver inflammation and fibrosis, whereas hepatocyte-derived MANF prevents hepatosteatosis [ 68].

Interestingly, MANF transgenic mice fed with a regular chow diet become obese (Fig. 2d). Starting from 4-month of age, both male and female MANF transgenic mice displayed significantly increased body weights compared with their WT littermates (Fig. 2e). This result is unlikely due to disruption of genes that are essential for energy homeostasis caused by random transgene insertion, as another MANF transgenic line showed a similar phenotype (Supplementary Fig. 2b). The increased body weight correlates with increased adiposity (Fig. 2f MANF Inhibits α-Synuclein Accumulation through Activation of Autophagic Pathways. Zhang JX, et al. Oxid Med Cell Longev, 2022. PMID 35847585, Free PMC Article Clark, R. I. & Walker, D. W. Role of gut microbiota in aging-related health decline: insights from invertebrate models. Cell. Mol. Life Sci. 75, 93–101 (2017).